Aesthetic News Blog

Understanding Botulinum Toxin Reconstitution

How Many Units??

If this phrase sounds familiar, you might find this blog on how to reconstitute botox and how many units are in certain volumes useful!




If you have 50 units of toxin (Bocouture or Botox Allergan) and this is reconstituted with 1.25ml of 0.9% sodium chloride then every 0.1 ml will hold the equivalent of 4 units of toxin. (0.05ml will be the equivalent of 2 units of toxin)


When using microfine insulin needles it is important to remember that the number of INSULIN units does NOT equal the same number of botox units.


For example, if you have a 0.5ml microfine insulin syringe it will be graded in values of 5 (eg 5,10,15,20 all the way to 50). When using this syringe each grading of 5 on the syringe is equal to 0.05ml of solution which equals 2 units of botox.


Therefore, if you wanted to place 4 units of botox you would be using 0.1ml which would be going down to gradings on this 0.5ml insulin syringe ie 0-5-10.


5 INSULIN units = 0.05 ml = 2 units of TOXIN*


10 INSULIN units = 0.1ml = 4 units of TOXIN*


It can sometimes be daunting, trying to get your head around dilutions and volumes, so you might find it helpful to print off the chart above and have it to hand while mixing and drawing up the units of toxin you need for treatment.


If you have any questions about foundation botulinum toxin, dermal filler courses, or further advanced training or you`re looking for some post-course support or advice please feel free to contact us at office@inspiredcosmetictraining.com or call 0141 638 4098 - we’re happy to help!


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22nd Nov  “Hands on” Practical Masterclass on Full Face Approach with Dr Usman Qureshi & Neauvia,

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27th Nov Advanced BOTOX® Training inc. Masseters & Hyperhidrosis,

6th Dec Foundation Botulinum Toxin & Filler Training,

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11th Dec Foundation Botulinum Toxin & Filler Training,

31st Jan Medical Chemical Peel & Microneedling Training, Glasgow

21st Mar The Scottish Aesthetic Conference 2026,




*When 50 units of toxin (bocouture or botox allergan only) are reconstituted with 1.25ml saline. These values DO NOT apply when using Azzalure.

Posted on Nov 18, 2025.


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